Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Manganese Size:1000g 2 Glucose 2 KH2PO4 1

2 Glucose 2 KH2PO4 1

Peptone 2 Yeast Extract 0

Usage above a 20 mM concentration in mammalian cell culture work is not recommended

Due to the low sodium chloride concentration Formedium™Casamino Acids are well suited for cultivation of yeast cells

5 Na2HPO4 0

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Manganese Size:1000g 2 Glucose 2 KH2PO4 1Manganese sulphate, MnSO4. H20 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Manganese or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store